Glutathione in semen extender modulates post thaw semen quality profiles, and antioxidant and oxidative stress profiles in mithun

Present study was designed to assess the effect of glutathione (GSH) on post thaw semen quality parameters (SQPs), sperm kinetic profiles, antioxidant and oxidative stress profiles cholesterol efflux in mithun. Ejaculates (25) were selected based biophysical for present experiment. Each sample split into four equal aliquots after dilution with Tris-citrate-glycerol (TCG) extender, viz. Group I (control), II, III IV contained 5, 10 15 mM GSH, respectively. Cryopreserved thawed samples analysed their motility (progressive forward bovine cervical mucus penetration test [BCMPT]), velocity by computer assisted analyser (CASA), viability, nuclear abnormalities, acrosomal integrity, plasma membrane integrities, enzymatic leakage biochemical (sperm stress) profiles. Study revealed a significant enhancement normalities, mucus), content reduction intracellular enzymes III. Moreover, intactness acrosome membranes protected significantly addition improvement extender containing GSH. These results clearly indicate that though cryopreservation mithun’s spermatozoa TCG comparable other species, inclusion GSH holds clear advantage over control 5 or The concludes supplementation can be effectively utilized reduce improve cascading beneficial effects cryopreserved mithun bull.